Error message

  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Notice: Trying to access array offset on value of type int in element_children() (line 6595 of /home2/rohscmet/public_html/includes/common.inc).
  • Deprecated function: implode(): Passing glue string after array is deprecated. Swap the parameters in drupal_get_feeds() (line 394 of /home2/rohscmet/public_html/includes/common.inc).
  • Deprecated function: The each() function is deprecated. This message will be suppressed on further calls in _menu_load_objects() (line 579 of /home2/rohscmet/public_html/includes/menu.inc).

UV-Visible Spectrophotometer

Make: Shimadzu

Model: UV-2450

Principle

Ultraviolet-Visible spectroscopy is working based on the principle of Beer Lambert’s law. It involves, absorbance is directly proportional to intensity of the colour and thickness of the medium.

Ultraviolet light: wavelengths between 190 and 400 nm

Visible light: wavelengths between 400 and 800 nm

Sample preparation

After dissolution of sample to adjust pH is 0.7-1.3 using buffer solutions, then add 1, 5-diphenylcarbazide reagent for colour development. The intensity of the coloured sample was measured by UV-Visible spectrophotometer.

Applications

For Hexavalent chromium Analysis

Strengths

·       Accurate determination

·       UV-VIS spectrophotometer is the basic instrument

·       Determination of maximum elements in periodic table

·       Cost of maintained is low

Limitations

·       Time taken for analysis

·       At a time one parameter only it measures

·       Colouring reagents are needed

How many decimal places does the unit give for results when used for absorbance?

This unit has readout to 3 decimal places. However this unit been discontinued and has been replaced with the 83059-10, which will also provide a readout 3 decimal points.

Is it a single or dual beam?

This is a dual beam instrument

What is the minimal volume of sample to read in the spectrophotometer?

This unit uses standard 10 mm cuvettes. If very small sample size are a concern, the thermo Nano-Drop uses as little as 0.5 microliters

 

workshop

DOWNLOADS
REQUEST QUOTE
Enquiry Form
CONTACT US
for more information
Dr. U. Rambabu,M.Phil.;Ph.D
Quality Manager		  
C-MET, IDA, Phase III, Cherlapally,
HCL Post, Hyderabad- 500051
Email: rambabu[at]cmet[dot]gov[dot]in;
rambabu32[at]rediffmail[dot]com
Ph :+91-40-27265673; 2720327;
Mobile: +91-9441435672;
9010984021
Fax: +91-40-27261658

 

Route Map

routemap

Hit Counter: